Functional convergence of signalling by GPI-anchored and anchorless forms of a salamander protein implicated in limb regeneration.

ABSTRACT: The GPI-anchor is an established determinant of molecular localisation and various functional roles have been attributed to it. The newt GPI-anchored three-finger protein (TFP) Prod1 is an important regulator of cell behaviour during limb regeneration, but it is unclear how it signals to the interior of the cell. Prod1 was expressed by transfection in cultured newt limb cells and activated transcription and expression of matrix metalloproteinase 9 (MMP9) by a pathway involving ligand-independent activation of epidermal growth factor receptor (EGFR) signalling and phosphorylation of extracellular regulated kinase 1 and 2 (ERK1/2). This was dependent on the presence of the GPI-anchor and critical residues in the α-helical region of the protein. Interestingly, Prod1 in the axolotl, a salamand

SUBMITTER: Blassberg RA 

PROVIDER: S-EPMC3001407 | biostudies-literature | 2011 Jan

REPOSITORIES: biostudies-literature