Impact of reference gene selection for target gene normalization on experimental outcome using real-time qRT-PCR in adipocytes.

ABSTRACT:

Background

With the current rise in obesity-related morbidities, real-time quantitative reverse transcription polymerase chain reaction (qRT-PCR) has become a widely used method for assessment of genes expressed and regulated by adipocytes. In order to measure accurate changes in relative gene expression and monitor intersample variability, normalization to endogenous control genes that do not change in relative expression is commonly used with qRT-PCR determinations. However, historical evidence has clearly demonstrated that the expression profiles of traditional control genes (e.g., β-actin, GAPDH, α-tubulin) are differentially regulated across multiple tissue types and experimental conditions.

Methodology/principal findings

Therefore, we validated six commonly used endoge

SUBMITTER: Ferguson BS 

PROVIDER: S-EPMC3001470 | biostudies-literature | 2010 Dec

REPOSITORIES: biostudies-literature