A preliminary X-ray study of human nucleoside diphosphate kinase A under oxidative conditions.

ABSTRACT: Nucleoside diphosphate kinase (NDPK) catalyzes transfer of the γ-phosphoryl group from a nucleoside triphosphate (NTP) to a nucleoside diphosphate. The high-energy phosphate for this reaction is usually supplied by ATP. NDPK plays a primary role not only in maintaining cellular pools of all NTPs but also in the regulation of important cellular processes. NDPK-A (or Nm23-H1), one of eight human NDPKs, acts as a metastasis suppressor for some tumour types. A recent study showed that homohexameric human NDPK-A is regulated in response to oxidative stress. The activity of NDPK-A is reduced, with a concomitant increase in the population of dimeric NDPK-A, under oxidative conditions. In this study, human NDPK-A has been crystallized under oxidative conditions and X-ray data have been collected t

SUBMITTER: Kim MS 

PROVIDER: S-EPMC3001656 | biostudies-literature | 2010 Nov

REPOSITORIES: biostudies-literature