Phenotypic and genotypic characterization of influenza virus mutants selected with the sialidase fusion protein DAS181.

ABSTRACT:

Background

influenza viruses (IFVs) frequently achieve resistance to antiviral drugs, necessitating the development of compounds with novel mechanisms of action. DAS181 (Fludase), a sialidase fusion protein, may have a reduced potential for generating drug resistance due to its novel host-targeting mechanism of action.

Methods

IFV strains B/Maryland/1/59 and A/Victoria/3/75 (H3N2) were subjected to >30 passages under increasing selective pressure with DAS181. The DAS181-selected IFV isolates were characterized in vitro and in mice.

Results

despite extensive passaging, DAS181-selected viruses exhibited a very low level of resistance to DAS181, which ranged between 3- and 18-fold increase in EC(50). DAS181-selected viruses displayed an attenuated phenotype in vitro, as

SUBMITTER: Triana-Baltzer GB 

PROVIDER: S-EPMC3001847 | biostudies-literature | 2011 Jan

REPOSITORIES: biostudies-literature