Discontinuous pH gradient-mediated separation of TiO2-enriched phosphopeptides.

ABSTRACT: Global profiling of phosphoproteomes has proven to be a great challenge due to the relatively low stoichiometry of protein phosphorylation and poor ionization efficiency in mass spectrometers. Effective, physiologically relevant, phosphoproteome research relies on the efficient phosphopeptide enrichment from complex samples. Immobilized metal affinity chromatography and titanium dioxide chromatography can greatly assist selective phosphopeptide enrichment. However, the complexity of resultant enriched samples is often still high, suggesting that further separation of enriched phosphopeptides is required. We have developed a pH gradient elution technique for enhanced phosphopeptide identification in conjunction with titanium dioxide chromatography. Using this process, we demonstrated its su

SUBMITTER: Park SS 

PROVIDER: S-EPMC3002755 | biostudies-literature | 2011 Feb

REPOSITORIES: biostudies-literature