Molecular characterization of two distinct monopartite begomoviruses infecting tomato in India.

ABSTRACT:

Background

Tomato leaf curl viruses, which are the members of the genus Begomovirus, have emerged as devastating pathogens worldwide causing huge economic losses and threatening production of crops like cassava, cotton, grain legumes and vegetables. Even though the ToLCV isolates from Northern India have been shown to possess bipartite genome (designated as DNA A and DNA B), those from Australia, Taiwan and Southern India have a single genomic component (DNA A). We describe here the genetic diversity of two isolates of monopartite Tomato leaf curl virus infecting tomato in two extreme regions (North and South) of Indian subcontinent.

Results

The rolling circle amplification (RCA) products obtained from symptomatic samples were digested, cloned and sequenced. The complete DNA

SUBMITTER: Pandey P 

PROVIDER: S-EPMC3002923 | biostudies-literature | 2010 Nov

REPOSITORIES: biostudies-literature