An ABC transporter mutation is correlated with insect resistance to Bacillus thuringiensis Cry1Ac toxin.

ABSTRACT: Transgenic crops producing insecticidal toxins from Bacillus thuringiensis (Bt) are commercially successful in reducing pest damage, yet knowledge of resistance mechanisms that threaten their sustainability is incomplete. Insect resistance to the pore-forming Cry1Ac toxin is correlated with the loss of high-affinity, irreversible binding to the mid-gut membrane, but the genetic factors responsible for this change have been elusive. Mutations in a 12-cadherin-domain protein confer some Cry1Ac resistance but do not block this toxin binding in in vitro assays. We sought to identify mutations in other genes that might be responsible for the loss of binding. We employed a map-based cloning approach using a series of backcrosses with 1,060 progeny to identify a resistance gene in the cotton pest

SUBMITTER: Gahan LJ 

PROVIDER: S-EPMC3002984 | biostudies-literature | 2010 Dec

REPOSITORIES: biostudies-literature