Mapping dynamic histone acetylation patterns to gene expression in nanog-depleted murine embryonic stem cells.

ABSTRACT: Embryonic stem cells (ESC) have the potential to self-renew indefinitely and to differentiate into any of the three germ layers. The molecular mechanisms for self-renewal, maintenance of pluripotency and lineage specification are poorly understood, but recent results point to a key role for epigenetic mechanisms. In this study, we focus on quantifying the impact of histone 3 acetylation (H3K9,14ac) on gene expression in murine embryonic stem cells. We analyze genome-wide histone acetylation patterns and gene expression profiles measured over the first five days of cell differentiation triggered by silencing Nanog, a key transcription factor in ESC regulation. We explore the temporal and spatial dynamics of histone acetylation data and its correlation with gene expression using supervised a

SUBMITTER: Markowetz F 

PROVIDER: S-EPMC3002996 | biostudies-literature | 2010 Dec

REPOSITORIES: biostudies-literature