DPI-ELISA: a fast and versatile method to specify the binding of plant transcription factors to DNA in vitro.

ABSTRACT:

Background

About 10% of all genes in eukaryote genomes are predicted to encode transcription factors. The specific binding of transcription factors to short DNA-motifs influences the expression of neighbouring genes. However, little is known about the DNA-protein interaction itself. To date there are only a few suitable methods to characterise DNA-protein-interactions, among which the EMSA is the method most frequently used in laboratories. Besides EMSA, several protocols describe the effective use of an ELISA-based transcription factor binding assay e.g. for the analysis of human NFκB binding to specific DNA sequences.

Results

We provide a unified protocol for this type of ELISA analysis, termed DNA-Protein-Interaction (DPI)-ELISA. Qualitative analyses with His-epitope tagg

SUBMITTER: Brand LH 

PROVIDER: S-EPMC3003642 | biostudies-literature | 2010 Nov

REPOSITORIES: biostudies-literature