Universal platform for quantitative analysis of DNA transposition.

ABSTRACT:

Background

Completed genome projects have revealed an astonishing diversity of transposable genetic elements, implying the existence of novel element families yet to be discovered from diverse life forms. Concurrently, several better understood transposon systems have been exploited as efficient tools in molecular biology and genomics applications. Characterization of new mobile elements and improvement of the existing transposition technology platforms warrant easy-to-use assays for the quantitative analysis of DNA transposition.

Results

Here we developed a universal in vivo platform for the analysis of transposition frequency with class II mobile elements, i.e., DNA transposons. For each particular transposon system, cloning of the transposon ends and the cognate transposa

SUBMITTER: Pajunen MI 

PROVIDER: S-EPMC3003695 | biostudies-literature | 2010 Nov

REPOSITORIES: biostudies-literature