Physiological expression of lens α-, β-, and γ-crystallins in murine and human corneas.

ABSTRACT:

Purpose

How corneal transparency is formed/maintained remains largely unclear. A group of enzymes which are referred to as enzymatic crystallins were proposed to contribute to corneal transparency in various animals. This study investigated whether the three classical lens crystallins, namely α-, β-, and γ-crystallins, exist in mouse and human corneas.

Methods

Mice, human tissues, and cultured corneal cells were studied. The expression of lens crystallins in corneas or in cultured corneal cells were detected at the mRNA level by quantitative reverse transcription-PCR (QRT-PCR) and at the protein level by immunohistochemistry or western blotting. To check the effect of exogenous factor on expression of lens crystallins, cultured corneal cells were challenged with lipopolysacc

SUBMITTER: Ren S 

PROVIDER: S-EPMC3003712 | biostudies-literature | 2010 Dec

REPOSITORIES: biostudies-literature