A biosynthetic strategy for re-engineering the Staphylococcus aureus cell wall with non-native small molecules.

ABSTRACT: Staphylococcus aureus (S. aureus) is a Gram-positive bacterial pathogen that has emerged as a major public health threat. Here we report that the cell wall of S. aureus can be covalently re-engineered to contain non-native small molecules. This process makes use of endogenous levels of the bacterial enzyme sortase A (SrtA), which ordinarily functions to incorporate proteins into the bacterial cell wall. Thus, incubation of wild-type bacteria with rationally designed SrtA substrates results in covalent incorporation of functional molecular handles (fluorescein, biotin, and azide) into cell wall peptidoglycan. These conclusions are supported by data obtained through a variety of experimental techniques (epifluorescence and electron microscopy, biochemical extraction, and mass spectrometry),

SUBMITTER: Nelson JW 

PROVIDER: S-EPMC3003768 | biostudies-literature | 2010 Dec

REPOSITORIES: biostudies-literature