Testing the IMEter on rice introns and other aspects of intron-mediated enhancement of gene expression.

ABSTRACT: In many eukaryotes, spliceosomal introns are able to influence the level and site of gene expression. The mechanism of this Intron Mediated Enhancement (IME) has not yet been elucidated, but regulation of gene expression is likely to occur at several steps during and after transcription. Different introns have different intrinsic enhancing properties, but the determinants of these differences remain unknown. Recently, an algorithm called IMEter, which is able to predict the IME potential of introns without direct testing, has been proposed. A computer program was developed for Arabidopsis thaliana and rice (Oryza sativa L.), but was only tested experimentally in Arabidopsis by measuring the enhancement effect on GUS expression of different introns inserted within otherwise identical plasmi

SUBMITTER: Morello L 

PROVIDER: S-EPMC3003800 | biostudies-literature | 2011 Jan

REPOSITORIES: biostudies-literature