Effect of collision energy optimization on the measurement of peptides by selected reaction monitoring (SRM) mass spectrometry.

ABSTRACT: Proteomics experiments based on Selected Reaction Monitoring (SRM, also referred to as Multiple Reaction Monitoring or MRM) are being used to target large numbers of protein candidates in complex mixtures. At present, instrument parameters are often optimized for each peptide, a time and resource intensive process. Large SRM experiments are greatly facilitated by having the ability to predict MS instrument parameters that work well with the broad diversity of peptides they target. For this reason, we investigated the impact of using simple linear equations to predict the collision energy (CE) on peptide signal intensity and compared it with the empirical optimization of the CE for each peptide and transition individually. Using optimized linear equations, the difference between predicted a

SUBMITTER: Maclean B 

PROVIDER: S-EPMC3005404 | biostudies-literature | 2010 Dec

REPOSITORIES: biostudies-literature