In vivo imaging of labelled endogenous β-actin mRNA during nucleocytoplasmic transport.

ABSTRACT: Export of messenger RNA occurs via nuclear pores, which are large nanomachines with diameters of roughly 120 nm that are the only link between the nucleus and cytoplasm. Hence, mRNA export occurs over distances smaller than the optical resolution of conventional light microscopes. There is extensive knowledge on the physical structure and composition of the nuclear pore complex, but transport selectivity and the dynamics of mRNA export at nuclear pores remain unknown. Here we developed a super-registration approach using fluorescence microscopy that can overcome the current limitations of co-localization by means of measuring intermolecular distances of chromatically different fluorescent molecules with nanometre precision. With this method we achieve 20-ms time-precision and at least 26-n

SUBMITTER: Grunwald D 

PROVIDER: S-EPMC3005609 | biostudies-literature | 2010 Sep

REPOSITORIES: biostudies-literature