A novel and efficient model of coronary artery ligation and myocardial infarction in the mouse.

ABSTRACT:

Rationale

coronary artery ligation to induce myocardial infarction (MI) in mice is typically performed by an invasive and time-consuming approach that requires ventilation and chest opening (classic method), often resulting in extensive tissue damage and high mortality. We developed a novel and rapid surgical method to induce MI that does not require ventilation.

Objective

the purpose of this study was to develop and comprehensively describe this method and directly compare it to the classic method.

Methods and results

male C57/B6 mice were grouped into 4 groups: new method MI (MI-N) or sham (S-N) and classic method MI (MI-C) or sham (S-C). In the new method, heart was manually exposed without intubation through a small incision and MI was induced. In the classic meth

SUBMITTER: Gao E 

PROVIDER: S-EPMC3005817 | biostudies-literature | 2010 Dec

REPOSITORIES: biostudies-literature