Dendritic cell and T cell responses in children with food allergy.

ABSTRACT:

Background

Food allergy (FA) and eosinophilic oesophagitis (EE) are increasingly common clinical problems. Dendritic cells (DCs) are key regulators of the sensitization and effector phases of allergic immune responses, but their role in these diseases is largely unknown.

Objective

To evaluate for alterations in the phenotype and function of DCs in children with IgE-mediated milk allergy or EE compared with their non-affected siblings.

Methods

Plasmacytoid DCs (pDCs) and myeloid DCs (mDCs) were prepared from peripheral blood of children with milk allergy (FA), EE, and non-affected siblings (CON). Purified pDCs and mDCs were cultured alone or with autologous CD4(+) lymphocytes. Cytokine levels in plasma, or culture supernatants following stimulation, were measured using

SUBMITTER: Frischmeyer-Guerrerio PA 

PROVIDER: S-EPMC3006008 | biostudies-literature | 2011 Jan

REPOSITORIES: biostudies-literature