Towards the construction of expressed proteomes using a Leishmania tarentolae based cell-free expression system.

ABSTRACT: The adaptation of organisms to a parasitic life style is often accompanied by the emergence of novel biochemical pathways absent in free-living organisms. As a result, the genomes of specialized parasitic organisms often code for a large number (>50%) of proteins with no detectable homology or predictable function. Although understanding the biochemical properties of these proteins and their roles in parasite biogenesis is the next challenge of molecular parasitology, analysis tools developed for free-living organisms are often inadequate for this purpose. Here we attempt to solve some of these problems by developing a methodology for the rapid production of expressed proteomes in cell-free systems based on parasitic organisms. To do so we take advantage of Species Independent Translationa

SUBMITTER: Kovtun O 

PROVIDER: S-EPMC3006200 | biostudies-literature | 2010 Dec

REPOSITORIES: biostudies-literature