Polymorphisms of HIV-2 integrase and selection of resistance to raltegravir.

ABSTRACT:

Background

Human Immunodeficiency Virus type 2 is naturally resistant to some antiretroviral drugs, restricting therapeutic options for patients infected with HIV-2. Regimens including integrase inhibitors (INI) seem to be effective, but little data on HIV-2 integrase (IN) polymorphisms and resistance pathways are available.

Materials and methods

The integrase coding sequence from 45 HIV-2-infected, INI-naïve, patients was sequenced and aligned against the ROD (group A) or EHO (group B) reference strains and polymorphic or conserved positions were analyzed.To select for raltegravir (RAL)-resistant variants in vitro, the ROD strain was cultured under increasing sub-optimal RAL concentrations for successive rounds. The phenotype of the selected variants was assessed using an M

SUBMITTER: Bercoff DP 

PROVIDER: S-EPMC3006360 | biostudies-literature | 2010 Nov

REPOSITORIES: biostudies-literature