Application of fractional mass for the identification of peptide-oligonucleotide cross-links by mass spectrometry.

ABSTRACT: A method has been developed to identify oligonucleotide-peptide heteroconjugates by accurate mass measurements using MS. The fractional mass (the decimal fraction mass value following the monoisotopic nominal mass) for peptides and oligonucleotides is different due to their differing molecular compositions. This property has been used to develop the general conditions necessary to differentiate peptides and oligonucleotides from oligonucleotide-peptide heteroconjugates. Peptides and oligonucleotides generated by the theoretical digestion of various proteins and nucleic acids were plotted as nominal mass versus fractional mass. Such plots reveal that three nucleotides cross-linked to a peptide produce enough change in the fractional mass to be recognized from non-cross-linked peptides at th

SUBMITTER: Pourshahian S 

PROVIDER: S-EPMC3008158 | biostudies-literature | 2008 Aug

REPOSITORIES: biostudies-literature