Evaluation of molecular assays for rapid detection of methicillin-resistant Staphylococcus aureus.

ABSTRACT: The diagnostic sensitivities of the BD GeneOhm and Cepheid Xpert assays were compared using culture on log-serial dilutions of well-characterized methicillin-resistant Staphylococcus aureus (MRSA) and non-MRSA strains and on nasal and groin swabs from patients with histories of MRSA carriage. The sensitivities of GeneOhm and Xpert were high at 10(3)-CFU/ml MRSA concentrations (92.3% and 96.3%, respectively) although decreased considerably (<35%) at a 1-log-lower concentration. Unexpectedly, both assays also detected select coagulase-negative staphylococci, which requires further evaluation.

SUBMITTER: Malhotra-Kumar S 

PROVIDER: S-EPMC3008470 | biostudies-literature | 2010 Dec

REPOSITORIES: biostudies-literature