Recombinant expression screening of P. aeruginosa bacterial inner membrane proteins.

ABSTRACT:

Background

Transmembrane proteins (TM proteins) make up 25% of all proteins and play key roles in many diseases and normal physiological processes. However, much less is known about their structures and molecular mechanisms than for soluble proteins. Problems in expression, solubilization, purification, and crystallization cause bottlenecks in the characterization of TM proteins. This project addressed the need for improved methods for obtaining sufficient amounts of TM proteins for determining their structures and molecular mechanisms.

Results

Plasmid clones were obtained that encode eighty-seven transmembrane proteins with varying physical characteristics, for example, the number of predicted transmembrane helices, molecular weight, and grand average hydrophobicity (GRAVY)

SUBMITTER: Madhavan V 

PROVIDER: S-EPMC3009615 | biostudies-literature | 2010 Nov

REPOSITORIES: biostudies-literature