The prognostic significance of whole blood global and specific DNA methylation levels in gastric adenocarcinoma.

ABSTRACT:

Background

Epigenetics, particularly DNA methylation, has recently been elucidated as important in gastric cancer (GC) initiation and progression. We investigated the clinical and prognostic importance of whole blood global and site-specific DNA methylation in GC.

Methods

Genomic DNA was extracted from the peripheral blood of 105 Omani GC patients at diagnosis. DNA methylation was quantified by pyrosequencing of global DNA and specific gene promoter regions at 5 CpG sites for CDH1, 7 CpG sites for p16, 4 CpG sites for p53, and 3 CpG sites for RUNX3. DNA methylation levels in patients were categorized into low, medium, and high tertiles. Associations between methylation level category and clinicopathological features were evaluated using χ(2) tests. Survival analyses were car

SUBMITTER: Al-Moundhri MS 

PROVIDER: S-EPMC3009731 | biostudies-literature | 2010 Dec

REPOSITORIES: biostudies-literature