Unraveling condition-dependent networks of transcription factors that control metabolic pathway activity in yeast.

ABSTRACT: Which transcription factors control the distribution of metabolic fluxes under a given condition? We address this question by systematically quantifying metabolic fluxes in 119 transcription factor deletion mutants of Saccharomyces cerevisiae under five growth conditions. While most knockouts did not affect fluxes, we identified 42 condition-dependent interactions that were mediated by a total of 23 transcription factors that control almost exclusively the cellular decision between respiration and fermentation. This relatively sparse, condition-specific network of active metabolic control contrasts with the much larger gene regulation network inferred from expression and DNA binding data. Based on protein and transcript analyses in key mutants, we identified three enzymes in the tricarboxy

SUBMITTER: Fendt SM 

PROVIDER: S-EPMC3010106 | biostudies-literature | 2010 Nov

REPOSITORIES: biostudies-literature