The effect of phosphate accumulation on metal ion homeostasis in Saccharomyces cerevisiae.

ABSTRACT: Much of what is currently understood about the cell biology of metals involves their interactions with proteins. By comparison, little is known about interactions of metals with intracellular inorganic compounds such as phosphate. Here we examined the role of phosphate in metal metabolism in vivo by genetically perturbing the phosphate content of Saccharomyces cerevisiae cells. Yeast pho80 mutants cannot sense phosphate and have lost control of phosphate uptake, storage, and metabolism. We report here that pho80 mutants specifically elevate cytosolic and nonvacuolar levels of phosphate and this in turn causes a wide range of metal homeostasis defects. Intracellular levels of the hard-metal cations sodium and calcium increase dramatically, and cells become susceptible to toxicity from the t

SUBMITTER: Rosenfeld L 

PROVIDER: S-EPMC3010241 | biostudies-literature | 2010 Sep

REPOSITORIES: biostudies-literature