Dysregulation of epithelial Na+ absorption induced by inhibition of the kinases TORC1 and TORC2.

ABSTRACT:

Background and purpose

Although the serum and glucocorticoid-inducible protein kinase 1 (SGK1) appears to be involved in controlling epithelial Na(+) absorption, its role in this physiologically important ion transport process is undefined. As SGK1 activity is dependent upon target of rapamycin complex 2 (TORC2)-catalysed phosphorylation of SGK1-Ser(422) , we have explored the effects of inhibiting TORC2 and/or TORC1 upon the hormonal control of Na(+) absorption.

Experimental approach

Na(+) absorption was quantified electrometrically in mouse cortical collecting duct cells (mpkCCD) grown to confluence on permeable membranes. Kinase activities were assessed by monitoring endogenous protein phosphorylation, with or without TORC1/2 inhibitors (TORIN1 and PP242) and the TORC1 in

SUBMITTER: Mansley MK 

PROVIDER: S-EPMC3010582 | biostudies-literature | 2010 Dec

REPOSITORIES: biostudies-literature