Methylglyoxal scavengers attenuate endothelial dysfunction induced by methylglyoxal and high concentrations of glucose.

ABSTRACT:

Background and purpose

Endothelial dysfunction is a feature of hypertension and diabetes. Methylglyoxal (MG) is a reactive dicarbonyl metabolite of glucose and its levels are elevated in spontaneously hypertensive rats and in diabetic patients. We investigated if MG induces endothelial dysfunction and whether MG scavengers can prevent endothelial dysfunction induced by MG and high glucose concentrations.

Experimental approach

Endothelium-dependent relaxation was studied in aortic rings from Sprague-Dawley rats. We also used cultured rat aortic and human umbilical vein endothelial cells. The MG was measured by HPLC and Western blotting and assay kits were used.

Key results

Incubation of aortic rings with MG (30 µM) or high glucose (25 mM) attenuated endothelium-depende

SUBMITTER: Dhar A 

PROVIDER: S-EPMC3010587 | biostudies-literature | 2010 Dec

REPOSITORIES: biostudies-literature