Evidence for CGRP re-uptake in rat dura mater encephali.

ABSTRACT:

Background and purpose

Calcitonin gene-related peptide (CGRP) is widely distributed in the trigeminovascular system and released from sensory fibres of the cranial dura mater upon noxious stimulation. Such release may be a mechanism underlying migraine headache. Based on data from guinea pig basilar artery preparations, we have here studied CGRP release and uptake in an organ preparation of the hemisected rat skull.

Experimental approach

CGRP release from the cranial dura was quantified by a commercial enzyme-linked immunoassay. CGRP was depleted using repetitive challenges of capsaicin. After incubating the tissue with CGRP for 20 min and extensive washing, another capsaicin challenge was performed. Immunohistochemistry was used to visualize CGRP immunofluorescence in dural

SUBMITTER: Gupta S 

PROVIDER: S-EPMC3010590 | biostudies-literature | 2010 Dec

REPOSITORIES: biostudies-literature