Fluorescent Citrine-IgG fusion proteins produced in mammalian cells.

ABSTRACT: Genetically encoded fluorescent antibodies are desirable for many applications in biotechnology, proteomics, microscopy, cell biology and molecular diagnostics, although efficient production of fluorescent IgGs in mammalian cells has been hampered by different and mutually incompatible secretion- and folding-requirements of antibodies and green fluorescent protein-derived fluorescent entities. Here, we show that this hurdle can be overcome by generating whole antibody fusions with Citrine, a modified yellow fluorescent protein that folds properly in the endoplasmic reticulum of mammalian cells. Applying optimized connector sequences, one or more Citrine molecules can be fused to different positions of IgGs without interfering with folding, secretion or function of the fusion proteins. Thes

SUBMITTER: Haas AK 

PROVIDER: S-EPMC3011219 | biostudies-literature | 2010 Nov-Dec

REPOSITORIES: biostudies-literature