Differential gene expression and clonal selection during cellular transformation induced by adhesion deprivation.

ABSTRACT:

Background

Anchorage independent growth is an important hallmark of oncogenic transformation. Previous studies have shown that when adhesion dependent fibroblasts were prevented from adhering to a substrate they underwent anoikis. In the present study we have demonstrated how anoikis resistant cells gain the transformation related properties with sequential selection of genes. We have proposed this process as a model system for selection of transformed cells from normal cells.

Results

This report demonstrates that some fibroblasts can survive during late stages of anoikis, at which time they exhibit transformation-associated properties such as in vitro colony formation in soft agar and in vivo subcutaneous tumour formation in nude mice. Cytogenetic characterisation of these

SUBMITTER: Jinka R 

PROVIDER: S-EPMC3012028 | biostudies-literature | 2010 Dec

REPOSITORIES: biostudies-literature