Global expression of cell surface proteins in embryonic stem cells.

ABSTRACT:

Background

Recent studies have shown that embryonic stem (ES) cells globally express most genes in the genome at the mRNA level; however, it is unclear whether this global expression is propagated to the protein level. Cell surface proteins could perform critical functions in ES cells, so determining whether ES cells globally express cell surface proteins would have significant implications for ES cell biology.

Methods and principal findings

The surface proteins of mouse ES cells were purified by biotin labeling and subjected to proteomics analysis. About 1000 transmembrane or secreted cell surface proteins were identified. These proteins covered a large variety if functional categories including signal transduction, adhesion and transporting. More over, mES cells promiscuou

SUBMITTER: Gu B 

PROVIDER: S-EPMC3012103 | biostudies-literature | 2010 Dec

REPOSITORIES: biostudies-literature