Prolonged application of high fluid shear to chondrocytes recapitulates gene expression profiles associated with osteoarthritis.

ABSTRACT:

Background

Excessive mechanical loading of articular cartilage producing hydrostatic stress, tensile strain and fluid flow leads to irreversible cartilage erosion and osteoarthritic (OA) disease. Since application of high fluid shear to chondrocytes recapitulates some of the earmarks of OA, we aimed to screen the gene expression profiles of shear-activated chondrocytes and assess potential similarities with OA chondrocytes.

Methodology/principal findings

Using a cDNA microarray technology, we screened the differentially-regulated genes in human T/C-28a2 chondrocytes subjected to high fluid shear (20 dyn/cm(2)) for 48 h and 72 h relative to static controls. Confirmation of the expression patterns of select genes was obtained by qRT-PCR. Using significance analysis of microarr

SUBMITTER: Zhu F 

PROVIDER: S-EPMC3012157 | biostudies-literature | 2010 Dec

REPOSITORIES: biostudies-literature