Peroxisome-generated hydrogen peroxide as important mediator of lipotoxicity in insulin-producing cells.

ABSTRACT:

Objective

Type 2 diabetes is a complex disease that is accompanied by elevated levels of nonesterified fatty acids (NEFAs), which contribute to β-cell dysfunction and β-cell loss, referred to as lipotoxicity. Experimental evidence suggests that oxidative stress is involved in lipotoxicity. In this study, we analyzed the molecular mechanisms of reactive oxygen species-mediated lipotoxicity in insulin-producing RINm5F cells and INS-1E cells as well as in primary rat islet cells.

Research design and methods

The toxicity of saturated NEFAs with different chain lengths upon insulin-producing cells was determined by MTT and propidium iodide (PI) viability assays. Catalase or superoxide dismutase overexpressing cells were used to analyze the nature and the cellular compartment of r

SUBMITTER: Elsner M 

PROVIDER: S-EPMC3012172 | biostudies-literature | 2011 Jan

REPOSITORIES: biostudies-literature