Expression and function of the K+ channel KCNQ genes in human arteries.

ABSTRACT:

Background and purpose

KCNQ-encoded voltage-gated potassium channels (K(v) 7) have recently been identified as important anti-constrictor elements in rodent blood vessels but the role of these channels and the effects of their modulation in human arteries remain unknown. Here, we have assessed KCNQ gene expression and function in human arteries ex vivo.

Experimental approach

Fifty arteries (41 from visceral adipose tissue, 9 mesenteric arteries) were obtained from subjects undergoing elective surgery. Quantitative RT-PCR experiments using primers specific for all known KCNQ genes and immunohistochemsitry were used to show K(v) 7 channel expression. Wire myography and single cell electrophysiology assessed the function of these channels.

Key results

KCNQ4 was expressed

SUBMITTER: Ng FL 

PROVIDER: S-EPMC3012405 | biostudies-literature | 2011 Jan

REPOSITORIES: biostudies-literature