Promoter polymorphism of MRP1 associated with reduced survival in hepatocellular carcinoma.

ABSTRACT:

Aim

to investigate the effect of the G-1666A polymorphism in the multidrug resistance related protein-1 (MRP1) on outcome of hepatocellular carcinoma (HCC).

Methods

a cohort of 162 patients with surgically resected HCC who received no postsurgical treatment until relapse was studied. Genotyping was performed by polymerase chain reaction-restriction fragment length polymorphism analysis. Electrophoretic mobility shift assay (EMSA) was used to evaluate the influence of the G-1666A polymorphism on the binding affinity of the MRP1 promoter with its putative transcription factors.

Results

Kaplan-Meier analysis showed that patients with GG homologues had a reduced 4-year disease-free survival compared with those carrying at least one A allele (P = 0.011). Multivariate Cox r

SUBMITTER: Zhao J 

PROVIDER: S-EPMC3012574 | biostudies-literature | 2010 Dec

REPOSITORIES: biostudies-literature