Effects of glial cell line-derived neurotrophic factor on cultured murine retinal progenitor cells.

ABSTRACT:

Purpose

Glial cell line-derived neurotrophic factor (GDNF) is neuroprotective of retinal neurons, and transduced retinal progenitor cells (RPCs) can deliver this cytokine for the treatment of retinal diseases, yet the potential effects of GDNF on RPCs have received little attention.

Methods

Murine RPCs were assessed under multiple conditions in the presence or absence of epidermal growth factor (EGF, 20 ng/ml) and/or GDNF (10 ng/ml) using a variety of techniques, including live-cell imaging, caspase-3 activity assay, whole genome microarray, quantitative polymerase chain reaction (qPCR), and western blotting.

Results

Live monitoring revealed that formation of initial aggregates resulted largely from the collision and adherence of dissociated RPCs, as opposed to clonal

SUBMITTER: Wang J 

PROVIDER: S-EPMC3012652 | biostudies-literature | 2010 Dec

REPOSITORIES: biostudies-literature