The essential role of clathrin-mediated endocytosis in the infectious entry of human enterovirus 71.

ABSTRACT: Little is currently known about the infectious entry process of human enterovirus 71 (HEV71) into host cells, which may represent potential anti-viral targeting sites. In this study a targeted small-interfering RNA (siRNA) screening platform assay was established and validated to identify and profile key cellular genes involved in processes of endocytosis, cytoskeletal dynamics, and endosomal trafficking essential for HEV71 infection. Screen evaluation was conducted via the expression of well characterized dominant-negative mutants, bioimaging studies (double-labeled immunofluorescence assays, transmission electron microscopy analysis), secondary siRNA-based dosage dependence studies, and drug inhibition assays. The infectious entry of HEV71 into rhabdomyosarcoma cells was shown to be sign

SUBMITTER: Hussain KM 

PROVIDER: S-EPMC3012988 | biostudies-literature | 2011 Jan

REPOSITORIES: biostudies-literature