The eEF1? subunit contacts RNA polymerase II and binds vimentin promoter region.

ABSTRACT: Here, we show that the eukaryotic translation elongation factor 1 gamma (eEF1?) physically interacts with the RNA polymerase II (pol II) core subunit 3 (RPB3), both in isolation and in the context of the holo-enzyme. Importantly, eEF1? has been recently shown to bind Vimentin mRNA. By chromatin immunoprecipitation experiments, we demonstrate, for the first time, that eEF1? is also physically present on the genomic locus corresponding to the promoter region of human Vimentin gene. The eEF1? depletion causes the Vimentin protein to be incorrectly compartmentalised and to severely compromise cellular shape and mitochondria localisation. We demonstrate that eEF1? partially colocalises with the mitochondrial marker Tom20 and that eEF1? depletion increases mitochondrial superoxide generation as well as the total levels of carbonylated proteins. Finally, we hypothesise that eEF1?, in addition to its role in translation elongation complex, is involved in regulating Vimentin gene by contacting both pol II and the Vimentin promoter region and then shuttling/nursing the Vimentin mRNA from its gene locus to its appropriate cellular compartment for translation.

SUBMITTER: Corbi N 

PROVIDER: S-EPMC3013090 | biostudies-literature | 2010 Dec

REPOSITORIES: biostudies-literature