Whole-blood gene expression profiling in ankylosing spondylitis shows upregulation of toll-like receptor 4 and 5.

ABSTRACT:

Objective

to identify differentially expressed genes in peripheral blood cells (PBC) of patients with ankylosing spondylitis (AS) relative to healthy controls and controls with systemic inflammation.

Methods

we investigated PBC samples of 16 patients with AS and 14 matched controls, in addition to systemic lupus erythematosus (SLE) and systemic sclerosis (SSc) samples utilizing Illumina Human Ref-8 BeadChips. Candidate genes were confirmed using quantitative PCR. Subsequently, these genes were also validated in a separate sample of 27 patients with AS [before and after anti-tumor necrosis factor (anti-TNF) treatment] and 27 matched controls.

Results

we identified 83 differentially expressed transcripts between AS patients and controls. This gene list was filtered thro

SUBMITTER: Assassi S 

PROVIDER: S-EPMC3014385 | biostudies-literature | 2011 Jan

REPOSITORIES: biostudies-literature