Analysis of transcript and protein overlap in a human osteosarcoma cell line.

ABSTRACT:

Background

An interesting field of research in genomics and proteomics is to compare the overlap between the transcriptome and the proteome. Recently, the tools to analyse gene and protein expression on a whole-genome scale have been improved, including the availability of the new generation sequencing instruments and high-throughput antibody-based methods to analyze the presence and localization of proteins. In this study, we used massive transcriptome sequencing (RNA-seq) to investigate the transcriptome of a human osteosarcoma cell line and compared the expression levels with in situ protein data obtained in-situ from antibody-based immunohistochemistry (IHC) and immunofluorescence microscopy (IF).

Results

A large-scale analysis based on 2749 genes was performed, correspo

SUBMITTER: Klevebring D 

PROVIDER: S-EPMC3014981 | biostudies-literature | 2010 Dec

REPOSITORIES: biostudies-literature