A novel epigenetic phenotype associated with the most aggressive pathway of bladder tumor progression.

ABSTRACT:

Background

Epigenetic silencing can extend to whole chromosomal regions in cancer. There have been few genome-wide studies exploring its involvement in tumorigenesis.

Methods

We searched for chromosomal regions affected by epigenetic silencing in cancer by using Affymetrix microarrays and real-time quantitative polymerase chain reaction to analyze RNA from 57 bladder tumors compared with normal urothelium. Epigenetic silencing was verified by gene re-expression following treatment of bladder cell lines with 5-aza-deoxycytidine, a DNA demethylating agent, and trichostatin A, a histone deacetylase inhibitor. DNA methylation was studied by bisulfite sequencing and histone methylation and acetylation by chromatin immunoprecipitation. Clustering was used to distinguish tumors wit

SUBMITTER: Vallot C 

PROVIDER: S-EPMC3014990 | biostudies-literature | 2011 Jan

REPOSITORIES: biostudies-literature