Combined actions of Na/K-ATPase, NCX1 and glutamate dependent NMDA receptors in ischemic rat brain penumbra.

ABSTRACT: Instrumental role of Na(+) and Ca(2+) influx via Na(+)/K(+) adenosine triphosphatase (Na(+)/K(+)-ATPase) and Na(+)/Ca(2+) exchanger 1 (NCX1) is examined in the N-Methyl-D-aspartate (NMDA) receptor-mediated pathogenesis of penumbra after focal cerebral ischemia. An experimental model of 3, 6, and 24 h focal cerebral ischemia by permanent occlusion of middle cerebral artery was developed in rats. The changes in protein expression of Na(+)/K(+)-ATPase and NCX1 as well as functional subunits of NMDA receptor 2A and 2B (NR2A and NR2B) in the penumbra were assessed using by quantitative immunoblottings. The most prominent changes of Na(+)/K(+)-ATPase (78±6%, n=4, (*)P<0.05) and NCX1 (144±2%, n=4, (*)P<0.05) in the penumbra were developed 24 h after focal cerebral ischemia. The expression of NR2A

SUBMITTER: Park S 

PROVIDER: S-EPMC3015038 | biostudies-literature | 2010 Sep

REPOSITORIES: biostudies-literature