Analysis of Escherichia coli O157 clinical isolates by multilocus sequence typing.

ABSTRACT:

Background

Although many strain typing methods exist for pathogenic Escherichia coli, most have drawbacks in terms of resolving power, interpretability, or scalability. For this reason, multilocus sequence typing (MLST) is an appealing alternative especially when applied to the typing of temporal and spatially separated isolates. This method relies on an unambiguous DNA sequence analysis of nucleotide polymorphisms in housekeeping genes and has shown a high degree of intraspecies discriminatory power for bacterial and fungal pathogens.

Results

Here we used the MLST method to study the genetic diversity among E. coli O157 isolates collected from humans from two different locations of USA over a period of several years (2000-2008). MLST analysis of 33 E. coli O157 patient isol

SUBMITTER: Rajkhowa S 

PROVIDER: S-EPMC3016269 | biostudies-literature | 2010 Dec

REPOSITORIES: biostudies-literature