Interaction of Sesbania mosaic virus movement protein with VPg and P10: implication to specificity of genome recognition.

ABSTRACT: Sesbania mosaic virus (SeMV) is a single strand positive-sense RNA plant virus that belongs to the genus Sobemovirus. The mechanism of cell-to-cell movement in sobemoviruses has not been well studied. With a view to identify the viral encoded ancillary proteins of SeMV that may assist in cell-to-cell movement of the virus, all the proteins encoded by SeMV genome were cloned into yeast Matchmaker system 3 and interaction studies were performed. Two proteins namely, viral protein genome linked (VPg) and a 10-kDa protein (P10) c v gft encoded by OFR 2a, were identified as possible interacting partners in addition to the viral coat protein (CP). Further characterization of these interactions revealed that the movement protein (MP) recognizes cognate RNA through interaction with VPg, which is c

SUBMITTER: Roy Chowdhury S 

PROVIDER: S-EPMC3016346 | biostudies-literature | 2011 Jan

REPOSITORIES: biostudies-literature