A mechanism for ramified rolling circle amplification.

ABSTRACT:

Background

Amplification of single-stranded DNA circles has wide utility for a variety of applications. The two-primer ramified rolling circle amplification (RAM) reaction provides exponential DNA amplification under isothermal conditions, creating a regular laddered series of double-stranded DNA products. However, the molecular mechanism of the RAM reaction remains unexplained.

Results

A RAM reaction model predicts exponential accumulation of a double-stranded DNA product size series, and product-size ratios, that are consistent with observed RAM reaction products. The mechanism involves generation of a series of increasing size intermediate templates; those templates produce RAM products and recursively generate smaller intermediate templates. The model allows prediction o

SUBMITTER: Beals TP 

PROVIDER: S-EPMC3017024 | biostudies-literature | 2010 Dec

REPOSITORIES: biostudies-literature