Identifying novel genes in C. elegans using SAGE tags.

ABSTRACT:

Background

Despite extensive efforts devoted to predicting protein-coding genes in genome sequences, many bona fide genes have not been found and many existing gene models are not accurate in all sequenced eukaryote genomes. This situation is partly explained by the fact that gene prediction programs have been developed based on our incomplete understanding of gene feature information such as splicing and promoter characteristics. Additionally, full-length cDNAs of many genes and their isoforms are hard to obtain due to their low level or rare expression. In order to obtain full-length sequences of all protein-coding genes, alternative approaches are required.

Results

In this project, we have developed a method of reconstructing full-length cDNA sequences based on short expr

SUBMITTER: Nesbitt MJ 

PROVIDER: S-EPMC3017025 | biostudies-literature | 2010 Dec

REPOSITORIES: biostudies-literature