Immunomodulative effects of mesenchymal stem cells derived from human embryonic stem cells in vivo and in vitro.

ABSTRACT:

Objective

Human embryonic stem cells (hESCs) have recently been reported as an unlimited source of mesenchymal stem cells (MSCs). The present study not only provides an identical and clinically compliant MSC source derived from hESCs (hESC-MSCs), but also describes the immunomodulative effects of hESC-MSCs in vitro and in vivo for a carbon tetrachloride (CCl(4))-induced liver inflammation model.

Methods

Undifferentiated hESCs were treated with Rho-associated kinase (ROCK) inhibitor and induced to fibroblast-looking cells. These cells were tested for their surface markers and multilineage differentiation capability. Further more, we analyzed their immune characteristics by mixed lymphocyte reactions (MLRs) and animal experiments.

Results

hESC-MSCs show a homogenous fib

SUBMITTER: Tan Z 

PROVIDER: S-EPMC3017412 | biostudies-literature | 2011 Jan

REPOSITORIES: biostudies-literature