A label-free differential quantitative mass spectrometry method for the characterization and identification of protein changes during citrus fruit development.

ABSTRACT:

Background

Citrus is one of the most important and widely grown commodity fruit crops. In this study a label-free LC-MS/MS based shot-gun proteomics approach was taken to explore three main stages of citrus fruit development. These approaches were used to identify and evaluate changes occurring in juice sac cells in various metabolic pathways affecting citrus fruit development and quality.

Results

Protein changes in citrus juice sac cells were identified and quantified using label-free shotgun methodologies. Two alternative methods, differential mass-spectrometry (dMS) and spectral counting (SC) were used to analyze protein changes occurring during earlier and late stages of fruit development. Both methods were compared in order to develop a proteomics workflow that could be

SUBMITTER: Katz E 

PROVIDER: S-EPMC3017515 | biostudies-literature | 2010 Dec

REPOSITORIES: biostudies-literature