Equivalence of conventionally-derived and parthenote-derived human embryonic stem cells.

ABSTRACT:

Background

As human embryonic stem cell (hESC) lines can be derived via multiple means, it is important to determine particular characteristics of individual lines that may dictate the applications to which they are best suited. The objective of this work was to determine points of equivalence and differences between conventionally-derived hESC and parthenote-derived hESC lines (phESC) in the undifferentiated state and during neural differentiation.

Methodology/principal findings

hESC and phESC were exposed to the same expansion conditions and subsequent neural and retinal pigmented epithelium (RPE) differentiation protocols. Growth rates and gross morphology were recorded during expansion. RTPCR for developmentally relevant genes and global DNA methylation profiling were us

SUBMITTER: Harness JV 

PROVIDER: S-EPMC3017547 | biostudies-literature | 2011 Jan

REPOSITORIES: biostudies-literature