Genome-wide mapping of RNA Pol-II promoter usage in mouse tissues by ChIP-seq.

ABSTRACT: Alternative promoters that are differentially used in various cellular contexts and tissue types add to the transcriptional complexity in mammalian genome. Identification of alternative promoters and the annotation of their activity in different tissues is one of the major challenges in understanding the transcriptional regulation of the mammalian genes and their isoforms. To determine the use of alternative promoters in different tissues, we performed ChIP-seq experiments using antibody against RNA Pol-II, in five adult mouse tissues (brain, liver, lung, spleen and kidney). Our analysis identified 38 639 Pol-II promoters, including 12 270 novel promoters, for both protein coding and non-coding mouse genes. Of these, 6384 promoters are tissue specific which are CpG poor and we find that on

SUBMITTER: Sun H 

PROVIDER: S-EPMC3017616 | biostudies-literature | 2011 Jan

REPOSITORIES: biostudies-literature